Figure 4.

ABCB1 and ABCC1 expression is not influenced by hypoxia on the protein and mRNA level. Cells were exposed to normoxia (21% oxygen) or hypoxia (1%). The abundance of ABCB1 and ABCC1 protein was examined by Western blot analysis after 16 h (in A), whereas abcb1 and abcc1 mRNA levels were analysed by rt-PCR after 4 h exposure (in B). Data are means ± SD values. Results from four (in A) or three (in B) different experiments are shown. Rt-PCR data were normalised to sdha that was evaluated as housekeeping gene.