Research
Neuronal precursor cell proliferation in the hippocampus after transient cerebral ischemia: a comparative study of two rat strains using stereological tools
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* Corresponding author: Jesper Kelsen jesperkelsen@hotmail.com
1 The Water and Salt Research Centre, University of Aarhus, DK-8000 Aarhus C, Denmark
2 Institute of Clinical Medicine, University Hospital of Aarhus, Brendstrupgaardsvej 100, DK-8200 Aarhus N, Denmark
3 Department of Neurosurgery, University Hospital of Copenhagen (Rigshospitalet), Blegdamsvej 9, DK-2100 Copenhagen Ø, Denmark
4 Department of Neuroscience and Pharmacology, The Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark
5 Department of Neurosurgery, University Hospital of Aarhus, Nørrebrogade 44, DK-8000 Aarhus C, Denmark
6 PET-centre, University Hospital of Aarhus, Nørrebrogade 44, DK-8000 Aarhus C, Denmark
7 Department of Cell Biology, Institute of Anatomy, Building 1230, University of Aarhus, DK-8000 Aarhus C, Denmark
8 Stereology and Electron Microscopy Research Laboratory and MIND Center, Danish Neuroscience Centre, University Hospital of Aarhus, Nørrebrogade 44, DK-8000 Aarhus C, Denmark
9 Neurobiology Research Unit, University Hospital of Copenhagen (Rigshospitalet), Building 9201, Blegdamsvej 9, DK-2100 Copenhagen Ø, Denmark
10 NEUROSEARCH A/S, Pederstrupvej 93, DK-2750 Ballerup, Denmark
Experimental & Translational Stroke Medicine 2010, 2:8 doi:10.1186/2040-7378-2-8
Published: 6 April 2010Additional files
Additional file 1:
Microloader dimensions. The diameters of the rounded microloaders were measured with CAST® software (Visiopharm A/S, Hørsholm, Denmark). Mean values ± SD are shown. One-way ANOVA with Bonferroni post hoc analysis was used for the comparison between the groups. * indicates p < 0.05. SD, Sprague-Dawley; SHR, spontaneously hypertensive rat; tMCAo, transient middle cerebral artery occlusion.
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Additional file 2:
Physiological parameters. Physiological parameters monitored before, during and after intraluminal tMCAo in SDs and SHRs. Mean values are presented ± SD. One-way ANOVA with Bonferroni post hoc analysis was used for the between group comparisons. * indicates p < 0.05, whereas p < 0.01 is marked with #. HR, heart rate; MABP, middle arterial blood pressure; Rectal Temp., rectal temperature; SD, Sprague-Dawley; SHR, spontaneously hypertensive rat; tMCAo, transient middle cerebral artery occlusion.
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Additional file 3:
Post-surgery development in animal body weight. All animals in the four groups were weighed daily (Figure 2D). There was a pronounced decrease in mean BW within the first four days after surgery in both strains. In general, the animals lost 12-15% of their preoperative weight. The weight curves tended to stabilize from Day 5 and on. Note that the SHRs had a lower mean BW although they were 6 weeks older than the SDs. SD sham and SD tMCAo are marked with "black triangles" and "black squares", whereas SHR sham and SHR tMCAo are represented with "black diamonds" and "black circles". BW, bodyweight; SDs, Sprague-Dawley rats; SHRs, spontaneously hypertensive rats; tMCAo, transient middle cerebral artery occlusion.
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